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The BIO-SPEC is designed to address the need for an open-source, cost-effective, and flexible bench-top parallel bioreactor system. The system is suited for batch, sequencing batch, and chemostat cultivation. It features thermoelectric condensers on the off-gas to avoid the need for a chiller, while ensuring stable long-term operation. Its modular and customisable design allows for various configurations and applications in microbiology and bioprocessing. The hardware is controlled at the core by a Raspberry Pi, offering a cost-effective alternative to commercial systems, with flexibility in headplate design, gas supply, and feeding strategies. This paper details the design, construction, and validation of the BIO-SPEC system, demonstrating its capabilities and potential for diverse research applications. By bridging the gap between high-cost commercial systems and do-it-yourself alternatives, the BIO-SPEC provides an accessible and reliable solution for advancing microbiological and bioprocessing research.

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BIO-SPEC: an open-source bench-top parallel bioreactor system

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